Monday, 6 February 2012

How to use RNA Marker (0.5 to 9 kb)

RNA Marker (0.5 to 9 kb) consists of RNA transcripts from 0.5 kb to 9 kb,in sizes of 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, and 9 kb.The markers can be denatured with standard procedures, and visualized on Northern blots with labeled lambda RNA sequence.Markers are provided at a concentration of 1mg/ml.Ethidium bromide staining will easily detect 4μg of DNA on a standard agarose gel.
Smaller quantities can be detected with GelStar Nucleic Acid Gel Stain or SYBR® Green II Gel Stain.
RNA Marker (0.5 to 9 kb) can be a difficult molecule to work with and is readily degraded by RNases that are found in a variety of environmental sources, including fingerprints. By minimizing nuclease contamination and ensuring the most stringent quality specifications, Ambion® products succeed in less-than-ideal conditions. This is referred to as "building robustness" into the products and continues to be a primary focus of our product development today.
Specification: 250ul (50ug)
Storage: DEPC treated H20/0.1 mM EDTA

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